Serial port control problem

[xp95]

Hi everybody,
I am working in project which aim to control 80 device through serial port.
First ,I made a simulation by Proteus and virtual port
Second, I used Micro C for Pic programming
Third, C# 2008 for computer interface

The simulation Succeeded but when i try to apply it on 8 devices in reality its failed ( LEDs open randomize)

Can any on tell me the reason?

Here all project files

http://www.4shared.com/file/KDAOA3nY/Final.html